An outbreak of abortion in Afshari sheep with probable involvement of Campylobacter fetus

Authors

  • Alireza Marefat Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
  • Masoumeh Saleh Department of Animal Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
  • Mohammad Taher Harkinezhad Department of Animal Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
  • Vahid Salmani Department of Animal Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Abstract:

BACKGROUND:Abortion is one of the most important factorsreducing lambing rate and consequently profitability of sheepfarms. In addition to financial losses, it is also important from azoonotic point of view. OBJECTIVES: The aim of this study wasto investigate bacterial abortifacient agents in an outbreak ofabortion occurring in Afshari sheep in the northwest of Zanjanprovince. METHODS:Vaginal swab samples were collected from217 Afshari ewes (129 samples were taken from aborted ewes, 3samples from ewes with crippled and deformed lambs, and 85samples from animals that had given birth to healthy lambs) fromreported flocks involved in outbreak. Swabs were examined byPCR assay to detect DNAfrom Coxiella burnetii, Chlamydophilaabortus, Salmonella enterica, Yersinia enterocolitica, Campylobacterfetus, Brucella ovis and Leptospira interrogans. RESULTS:Based on the results, only DNA of Campylobacter was detectedin the samples. A 266 bp fragment specific for Campylobacterwas amplified from 51.52% and 34.12% samples belonging toaborted and non-aborted ewes, respectively. CONCLUSIONS:Significant presence of the bacterium in aborted ewes (p

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Journal title

volume 7  issue 1

pages  51- 56

publication date 2013-04-01

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